Updated November 2024
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Westmoreland, D. E., Feliciano, P. R., Kang, G., Cui, C., Kim, A., Stubbe, J., Nocera, D. G., Drennan, C. L. 2.6-Å resolution cryo-EM structure of a class Ia ribonucleotide reductase trapped with mechanism-based inhibitor N3CDP. Proc. Natl. Acad. Sci. Proc. Full text.
Biester, A., Grahame, David A., Drennan, C.L. (2024). Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy. Proc. Natl. Acad. Sci. Proc. Full text.
Adak, S., Ye, N., Calderone, L.A., Duan, M., Lubeck, W., Schäfer, R.J.B., Lukowski, A.L., Houk, K.N., Pandelia, M.E., Drennan, C.L., Moore, B.S. (2024). A single diiron enzyme catalyses the oxidative rearrangement of tryptophan to indole nitrile. Nature Chemistry. Full text.
Funk, M.A., Zimanyi, C.M., Andree, G.A., Hamilos, A.E., Drennan, C.L. (2024). How ATP and dATP Act as Molecular Switches to Regulate Enzymatic Activity in the Prototypical Bacterial Class Ia Ribonucleotide Reductase. ACS Biochemistry. Full text.
Coco, L.B., Toci, E.M., Chen, P.Y., Drennan, C.L., & Freel Meyers, C.L. (2024). Potent Inhibition of E. coli DXP Synthase by a gem-Diaryl Bisubstrate Analog. ACS infectious diseases. Full text.
Vasquez, S., Marquez, M.D., Brignole, E.J., Vo, A., Kong, S., Park, C., Perlstein, D.L., and Drennan, C.L. (2023) Structural and biochemical investigations of a HEAT-repeat protein involved in the cytosolic iron-sulfur cluster assembly pathway, Commun Biol. Full text.
Vaccaro, F.A., Faber, D.A., Andree, G.A., Born, D., Kang, G., Fonseca, D.R., Jost, M., and Drennan, C.L. (2023) Structural insight into G-protein chaperone-mediated maturation of a bacterial adenosylcobalamin-dependent mutase, J. Biol. Chem. Full text.
Cui, C., Song, D.Y., Drennan, C.L., Stubbe*, J., and Nocera*, D.G. (2023) Radical Transport Facilitated by a Proton Transfer Network at the Subunit Interface of Ribonucleotide Reductase, J. Am. Chem. Soc. Full text.
Vaccaro, F.A., Born, D.A., and Drennan*, C.L. (2023) Structure of metallochaperone in complex with the cobalamin-binding domain of its target mutase provides insight into cofactor delivery, Proc. Natl. Acad. Full text.
Levitz, T. S. and Drennan, C. L. (2022) Starting a new chapter on class Ia ribonucleotide reductases. Current Opinion in Structural Biology. 77:102489. Full text.
Biester, A., Marcano-Delgado, A.N. and Drennan, C. L. (2022) Structural Insights into Microbial One-Carbon Metabolic Enzymes Ni–Fe–S-Dependent Carbon Monoxide Dehydrogenases and Acetyl-CoA Synthases. Biochemistry. Full text. PMCID# PMC9782325.
Sankari, S., Babu, V.M.P., Bian, K., Alhhazmi,A., Andorfer, M.C., Avalos, D.M., Smith, T. A., Yoon, K., Drennan, C. L., Yaffe, M.B., Lourido , S. and Walker , G.C. (2022) A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria. Nature Microbiology. https://news.mit.edu/2022/heme-plant-molecule-iron-0811.
Levitz, T. S., Weckener, M., Fong, I., Naismith, J. H., Drennan, C. L., Brignole, E. J., Clare, D. K., Darrow, M. C. (2022) Approaches to Using the Chameleon: Robust, Automated, Fast-Plunge cryoEM Specimen Preparation. Front. Mol. Biosci. 9.
A rapid and sensitive assay for quantifying the activity of both aerobic and anaerobic ribonucleotide reductases acting upon any or all substrates. PLoS One. 17(6):e0269572. PMCID: PMC9176816.
Vaccaro, F. A., Drennan, C. L. (2022) The role of nucleoside triphosphate hydrolase metallochaperones in making metalloenzymes. Metallomics. 14(6). PMCID: PMC9164220. Full text.
Ulrich, E., Drennan, C. L. (2022) The Atypical Cobalamin-Dependent SAdenosyllMethionine Nonradical Methylase TsrM and Its Radical Counterparts. J. Am. Chem. Soc. 144(13):5673-5684. PMCID# PMC8992657.
Biester, A., Dementin, S., Drennan, C. L. (2022) Visualizing the gas channel of a monofunctional carbon monoxide dehydrogenase. J. Inorg. Biochem. 230, 111774. PMCID: PMC9093221. Full text.
Ohmer, C. J., Dasgupta, M., Patwardhan, A., Bogacz, I., Kaminsky, C., Doyle, M. D., Chen, P. Y. T., Keable, S. M., Makita, H., Simon, P.S., Massad, R., Fransson, T., Chatterjee, R., Bhowmick, A., Paley, D., Moriarty, N. W., Brewster, A. S., Gee, L. B., Alonso-Mori, R., Moss, F., Fuller, F. D., Batyuk, A., Sauter, N. K., Bergmann, U., Drennan, C. L., Yachandra, V. K., Yano, J., Kern, J.F., Ragsdale, S. W. (2022) XFEL serial crystallography reveals the room temperature structure of methyl-coenzyme M reductase. J. Inorg. Biochem. 230, 111768. Full text.
Bridwell-Rabb*, J., Li, B., Drennan, C.L. (2022) Cobalamin-dependent Radical S-adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions, ACS Bio. & Med. Chem. 2(3), 173-186. https://doi.org/10.1021/acsbiomedchemau.1c00051. PMCID: PMC9204698
Levitz, T.S., Brignole, E.J., Fong, I., Darrow, M.C., and Drennan, C.L. (2022) Effects of chameleon dispense-to-plunge speed on particle concentration, complex formation, and final resolution: A case study using the Neisseria gonorrhoeae ribonucleotide reductase inactive complex. J. Struct. Biol. 214, 107825.
Andorfer, M.C., Backman, L.R.F., Li, P.L., Ulrich, E.C., Drennan, C.L., (2021) Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein. J. Biol. Chem. 297(6). Full text.
Feliciano PR, Carroll KS, Drennan* CL. (2021). Crystal structure of the [4Fe–4S] cluster-containing adenosine-5′-phosphosulfate reductase from Mycobacterium tuberculosis. ACS Omega 6:13756-13765. Full text here.
Andorfer* MC, Drennan CL. (2021). Fixing nature’s carbon inefficiencies. Joule 5:765-767. Full text.
Dawson CD, Irwin SM, Backman LRF, Le C, Wang JX, Vennelakanti V, Yang Z, Kulik* HJ, Drennan* CL, Balskus* EP. (2021). Molecular basis of C-S bond cleavage in the glycyl radical enzyme isethionate sulfite-lyase. Cell Chem. Biol. Online Now. Full text.
Knox HL, Chen PY-T, Blaszczyk AJ, Mukherjee A, Grove TL, Schwalm EL, Wang B, Drennan* CL, Booker* SJ. (2021). Structural basis for non-radical catalysis by TsrM, a radical SAM methylase. Nat. Chem. Biol. 17:485-491. Full text.
Jonnalagadda R, Del Rio Flores A, Cai W, Mehmood R, Narayanamoorthy M, Ren C, Zaragoza JPT, Kulik HJ, Zhang W, Drennan* CL. (2020). Biochemical and crystallographic investigations into isonitrile formation by a non-heme iron-dependent oxidase/decarboxylase, J. Biol. Chem. 296:100231. Full text.
Cui C, Greene* BL, Kang G, Drennan CL, Stubbe* J, Nocera* DG. (2020) Gated proton release during radical transfer at the subunit interface of ribonucleotide reductase. J. Am. Chem. Soc. 143:176-183. Full text.
Cohen SE, Brignole EJ, Wittenborn EC, Can M, Thompson S, Ragsdale SW, Drennan* CL. (2020) Negative-stain electron microscopy reveals dramatic structural rearrangements in Ni-Fe-S-dependent carbon monoxide dehydrogenase/acetyl-CoA synthase. Structure, 29:43-49.E3. Text at Structure.
Cohen SE, Can M, Wittenborn EC, Hendrickson RA, Ragsdale SW, Drennan* CL. (2020). Crystallographic characterization of the carbonylated A-cluster in carbon monoxide dehydrogenase/acetyl-CoA synthase. ACS Catal. 10:9741-9746. Text at ACS Catal.
Reinhardt CR, Li P, Kang G, Stubbe J, Drennan CL, Hammes-Schiffer* S. (2020) Conformational motions and water networks at the α/β interface in E. coli ribonucleotide reductase. J. Am. Chem. Soc. 142:13768-13778. Full text.
Greene BL, Kang G, Cui C, Bennati M, Nocera DG, Drennan CL, Stubbe* J. (2020) Ribonucleotide reductases: Structure, chemistry, and metabolism suggest new therapeutic targets. Annu. Rev. Biochem. 8:45-75. Full text.
Rizzolo K, Weitz AC, Cohen S, Drennan CL, Hendrich MP, Elliott* SJ. (2020). A stable ferryl porphyrin at the active site of Y463M BthA. J. Am. Chem. Soc. 142:11978-11982. Text at JACS.
Wittenborn EC, Guendon C, Merrouch M, Benvenuti M, Fourmond V, Léger C, Drennan* CL, Dementin* S. (2020) The solvent-exposed Fe–S D-cluster contributes to oxygen-resistance in Desulfovibrio vulgaris Ni–Fe carbon monoxide dehydrogenase. ACS Catal. 10: 7328-7335. Text at ACS Catal.
Kang G, Taguchi AT, Stubbe* J, Drennan* CL. (2020) Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex. Science 368:424-427. Full text; PDF
Bollenbach M, Ortega M, Orman M, Drennan CL, Balskus* EP. (2020) Discovery of a cyclic choline analog that inhibits anaerobic choline metabolism by human gut bacteria. ACS Medicinal Chemistry Letters 11:1980-1985. Full text
Backman‡ LRF, Huang‡ YY, Andorfer MC, Gold B, Raines RT, Balskus* EP, Drennan* CL. (2020) Molecular basis for catabolism of the abundant metabolite trans-4-hydroxy-L-proline by a microbial glycyl radical enzyme. eLife 9:e51420. Full text
Feliciano* PR, Drennan* CL. (2019) Structural and biochemical investigations of the [4Fe‑4S] cluster–containing fumarate hydratase from Leishmania major. Biochemistry 58:5011-5021. Full text
Wittenborn EC, Cohen SE, Merrouch M, Léger C, Fourmond V, Dementin* S, Drennan* CL. (2019) Structural insight nto metallocofactor maturation in carbon monoxide dehydrogenase. J. Bio. Chem. 294:13017-13026. Full text at JBC
Bowman SEJ, Backman LRF, Bjork RE, Andorfer MA, Yori S, Caruso A, Stultz CM, Drennan* CL. (2019) Solution structure and biochemical characterization of a spare part protein that restores activity to an oxygen-damaged glycyl radical enzyme. J. Biol. Inorg. Chem. 24:817-829. Full text at JBIC
Chen PY-T, DeColli AA, Freel Meyers* CL, Drennan* CL. (2019) X-ray crystallography–based structural elucidation of enzyme-bound intermediates along the 1-deoxy-D-xylulose 5-phosphate synthase reaction coordinate. J. Bio. Chem. 294:12405-12414. Full text at JBC
Rizzolo K, Cohen SE, Weitz AC, López Muñoz MM, Hendrich MP, Drennan CL, Elliott* SJ. (2019) A widely distributed diheme enzyme from Burkholderia that displays an atypically stable bis-Fe(IV) state. Nat. Commun. 10:1101. Full text at Nat. Commun.
Feliciano PR, Drennan* CL, Nonato* MC. (2019) Crystal structures of fumarate hydratases from Leishmania major in a complex with inhibitor 2-thiomalate. ACS Chem. Biol. 14(2):266-275. Full text at ACS Chem Biol
Chen‡ PY-T, Li‡ B, Drennan* CL, Elliott* SJ. (2019) A reverse TCA cycle 2-oxoacid:ferredoxin oxidoreductase that makes C–C bonds from CO₂. Joule 3(2):595-611. Full text
Grell TAJ, Bell BN, Nguyen C, Dowling DP, Bruender NA, Bandarian V, Drennan* CL. (2018) Crystal structure of AdoMet radical enzyme 7-carboxy-7-deazaguanine synthase from Escherichia coli suggests how modifications near [4Fe-4S] cluster engender flavodoxin specificity. Protein Sci. 28:202–215 Full text
Grell TAJ, Kincannon WM, Bruender NA, Blaesi EJ, Kreb* C, Bandarian* V, Drennan* CL. (2018) Structural and spectroscopic analyses of the sporulation killing factor biosynthetic enzyme SkfB, a bacterial AdoMet radical sactisynthase. J. Bio. Chem. 293:17349-17361. Full text at J Bio Chem
Wittenborn EC, Merrouch M, Ueda C, Fradale L, Léger C, Fourmond V, Pandelia M-E, Dementin* S, Drennan* CL. (2018) Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase. eLife 7:e39451. Full text at eLife
Ryan KS, Drennan CL. (2018) Metalloproteins in natural product biosynthetic pathways. Nat. Prod. Rep. 35:616-614. Full text
Bridwell-Rabb* J, Grell TAJ, Drennan* CL. (2018) A rich man, poor man story of S-adenosylmethionine and cobalamin revisited. Ann. Rev. of Biochem. 87:555-584. DOI: 10.1146/annurev-biochem-062917-012500
Harris NC, Born DA, Cai W, Huang Y, Martin J, Khalaf R, Drennan CL, Zheng* W. (2018) Isonitrile formation by a non-heme iron(II)-dependent oxidase/decarboxylase. Angew. Chemie Int. Ed. 57:9707-9710. Full text
Toshiki TG, Bowman SEJ, Zygiel EM, Drennan* CL, Nolan* EM. (2018) Biophysical examination of the calcium-modulated nickel-binding properties of human calprotectin reveals conformational change in the EF-hand domains and His3Asp site. Biochemistry 57:4155-4164. Full text at Biochemistry
Grell‡ TAJ, Young‡ AP, Drennan* CL, Bandarian* V. (2018) Biochemical and structural characterization of a Schiff base in the radical-mediated biosynthesis of 4-demethylwyosine by TYW1. J. Am. Chem. Soc. 140:6842-6852. Full text at JACS
Chen‡ PY-T, Funk‡ MA, Brignole EJ, Drennan* CL. (2018) Disruption of an oligomeric interface prevents allosteric inhibition of Escherichia coli class Ia ribonucleotide reductase. J. Biol. Chem. 293:10404-10412. Full text at JBC
Chen PY-T, Aman H, Can M, Ragsdale SW, Drennan* CL. (2018) Binding site for coenzyme A revealed in the structure of pyruvate:ferredoxin oxidoreducatase from Moorella thermoacetica. Proc. Natl. Acad. Sci. U.S.A. Full text at PNAS
Brignole‡ EJ, Tsai‡ KL, Chittuluru J, Li H, Aye Y, Penczek PA, Stubbe* J, Drennan* CL, Asturias* F. (2018) 3.3-Å resolution cryo-EM structure of human ribonucleotide reductase with substrate and allosteric regulators bound. eLife 7:e31502. Full text at eLife
Born‡ DA, Ulrich‡ EC, Ju KS, Peck SC, van der Donk* WA, Drennan* CL. (2017) Structural basis for methylphosphonate biosynthesis. Science 358(6368):1336-1339. Full text at Science
Backman LRF, Funk MA, Dawson CD, Drennan* CL. (2017) New tricks for the glycyl radical enzyme family. Critical Reviews In Biochemistry & Molecular Biology 52(6):674-695. Full text
Shisler K, Hutcheson R, Horitani M, Duschene K, Crain A, Byer A, Shepard E, Rasmussen A, Yang J, Broderick W, Vey J, Drennan CL, Hoffman B, Broderick* J. (2017) Monovalent cation activation of the radical SAM enzyme pyruvate formate-lyase activating enzyme. J. Am. Chem. Soc. 139(34):11803-11813. Full text at J. Am. Chem. Soc.
Bridwell-Rabb‡ J, Zhong‡ A, Sun HG, Drennan* CL, Liu* H-w. (2017) A B12-dependent radical SAM enzyme involved in oxetanocin-A biosynthesis. Nature 544:322-326. Full text at Nature
Kung* Y, Drennan CL. (2017) One-carbon chemistry of nickel-containing carbon monoxide dehydrogenase and acetyl-CoA synthase. In The Biological Chemistry of Nickel, D. Zamble, M. Rowinska-Zyrek, H. Kozlowski, eds., Royal Society of Chemistry, London, pp. 121-148. DOI: 10.1039/9781788010580-00121
Cory SA, Van Vranken JG, Brignole EJ, Patra S, Winge DR, Drennan CL, Rutter J, Barondeau* DP. (2017) Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions, Proc. Natl. Acad. Sci. U.S.A. . Full text at PNAS
Lin Q, Parker MJ, Taguchi AT, Ravichandran K, Kim A, Kang G, Drennan* CL, Stubbe* J. (2017) Glutamate 52-β at the α/β subunit interface of E. coli class Ia ribonucleotide reductase is essential for conformational gating of radical transfer. J. Biol. Chem. 292:9229-9239. Full text at J. Biol. Chem.
Nakashige‡ TG, Zygiel‡ EM, Drennan* CL, Nolan* EM. (2017) Nickel sequestration by the host-defense protein human calprotectin. J. Am. Chem. Soc. 139:8828-8836. Full text at JACS
Padmanabhan* S, Jost* M, Drennan* CL, Elias-Arnanz* M. (2017) A new facet of vitamin B12: Gene regulation by cobalamin-based photoreceptors. Ann. Rev. Biochem. 86:485-514. Full text at Ann. Rev. Biochem.
Bridwell-Rabb* J, Drennan* CL. (2017) Vitamin B12 in the spotlight again. Curr. Op. Chem. Biol. 37:63-70. Full text at ScienceDirect
Bruender NA, Grell TAJ, Dowling DP, McCarty RM, Drennan CL, Bandarian* V. (2017) 7-carboxy-7-deazaguanine synthase: A radical S-adenosyl-L-methionine enzyme with polar tendencies. J. Am. Chem. Soc. 139:1912-1920. Full text at JACS
Bridwell-Rabb J, Kang G, Zhong A, Liu H-w, Drennan* CL. (2016) An HD-domain phosphohydrolase active site tailored for oxetanocin-A biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 113:12432-12437. Full text at PNAS
Wittenborn EC, Jost M, Wei Y, Stubbe* J, Drennan* CL. (2016) Structure of the catalytic domain of the class I polyhydroxybutyrate synthase from Cupriavidus necator. J. Biol. Chem. 291:25264-25277. Full text at JBC
Dowling* DP, Kung Y, Croft AK, Taghizadeh K, Kelly WL, Walsh CT, Drennan* CL. (2016) Structural elements of an NRPS cyclization domain and its intermodule docking domain. Proc. Natl. Acad. Sci. U.S.A. Full text
Dowling DP, Miles ZD, Köhrer C, Maiocco SJ, Elliott SJ, Bandarian V, Drennan* CL. (2016) Molecular basis of cobalamin-dependent RNA modification, Nucleic Acids Research 44(20):9965-9976. Full text at NAR
Bodea‡ S, Funk‡ MA, Balskus* EP, Drennan* CL. (2016) Molecular basis of C–N bond cleavage by the glycyl radical enzyme choline trimethylamine-lyase. Cell Chem. Biol. 23:1206-1216. Full text at Cell Chemical Biology
Feliciano PR, Drennan* CL, Nonato* MC. (2016) Crystal structure of an Fe-S cluster–containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold. Proc. Natl. Acad. Sci. U.S.A. Full text
Gibson MI, Chen PY-T, Drennan* CL. (2016) A structural phylogeny for understanding 2-oxoacid oxidoreductase function. Current Opinion in Structural Biology 41:54-61. Full text at Science Direct
McLaughlin MI, Lanz ND, Goldman PJ, Lee K-H, Booker SJ, Drennan* CL. (2016) Crystallographic snapshots of sulfur insertion by lipoyl synthase. Proc. Natl. Acad. Sci. U.S.A. Full text
Oyala PH, Ravichandran KR, Funk MA, Stucky PA, Stich TA, Drennan* CL, Britt* RD, Stubbe* J. (2016) Biophysical characterization of fluorotyrosine probes site-specifically incorporated into enzymes: E. coli ribonucleotide reductase as an example. J. Am. Chem. Soc. 138(25):7951-7964. Full text at JACS
Bridwell-Rabb* J, Drennan* CL. (2016) A radically unexpected mechanism. Science 351(6279):1266-1267. Full text at Science, PDF
Meyer PA, et al. (2016) Data publication with the structural biology data grid supports live analysis. Nature Communications 7:10882. Full text at Nature
Bowman‡ SEJ, Bridwell-Rabb‡ J, Drennan CL. (2016) Metalloprotein crystallography: More than a structure. Accounts of Chemical Research 49(4):695-702. ACS Editor’s Choice, Full text at ACS
Chen F, Tang Q, Bian K, Humulock ZT, Yang X, Jost M, Drennan CL, Essigman JM, Li* D. (2016) Adaptive response enzyme AlkB preferentially repairs 1-methylguanine and 3-methylthymine adducts in double-stranded DNA. Chem. Res. Toxicol. 29(4):687-693. Full text at ACS
Chen PY-T, Wittenborn EC, Drennan* CL. (2016) Nitrogen fixation: Waltzing around cofactors. eLife 5:e13977. Full text at eLife
Zimanyi CM, Chen PY-T, Kang G, Funk MA, Drennan* CL. (2016) Molecular basis for allosteric specificity regulation in class Ia ribonucleotide reductase from Escherichia coli. eLife 5:e07141. Full text at eLife
Gibson MI, Chen PY-T, Johnson AC, Pierce E, Can M, Ragsdale SW, Drennan* CL. (2016) One-carbon chemistry of oxalate oxidoreductase captured by X-ray crystallography. Proc. Natl. Acad. Sci. U.S.A. 113(2):320-325. Full text at PNAS
Ando N, Li H, Brignole EJ, Thompson S, McLaughlin MI, Page JE, Asturias FJ, Stubbe J, Drennan* CL. (2016) Allosteric inhibition of human ribonucleotide reductase by dATP entails the stabilization of a hexamer. Biochemistry 55(2):373-381. Full text at ACS
Jost M, Born DA, Cracan VF, Banerjee R, Drennan* CL. (2015) Structural basis for substrate specificity in adenosylcobalamin-dependent isobutyryl-CoA mutase and related acyl-CoA mutases. J. Biol. Chem. 290:26882-26898. Full text at JBC
Jost M, Fernández-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY-T, Kang G, Padmanabhan* S, Elías-Arnanz* M, Drennan* CL. (2015) Structural basis for gene regulation by a B12-dependent photoreceptor. Nature 526:536-541. Full text
Jost M, Simpson JH, Drennan* CL. (2015) The transcription factor CarH safeguards use of adenosylcobalamin as a light sensor by altering the photolysis products. Biochemistry 54:3231-3234. Full text at ACS Publications
Bewley KD, Dey M, Bjork RE, Mitra S, Chobot SE, Drennan CL, Elliott* SJ. (2015) Rheostat re-wired: Alternate hypotheses for the control of thioredoxin reduction potentials. PLoS One 10:e0122466. Full text at PLOS ONE
Mattaini KR, Brignole EJ, Kini M, Davidson SM, Fiske BP, Drennan CL, Vander Heiden* MG. (2015) An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation. Cancer & Metabolism 3:5. Full text at Cancer & Metabolism
Funk MA, Marsh ENG, Drennan* CL. (2015) Substrate-bound structures of benzylsuccinate synthase reveal how toluene is activated in anaerobic hydrocarbon degradation. J. Biol. Chem. 290:22398-22408. Full text at JBC
Gibson MI, Brignole EJ, Pierce E, Can M, Ragsdale SW, Drennan* CL. (2015). The structure of an oxalate oxidoreductase provides insight into microbial 2-oxoacid metabolism. Biochemistry 54(26):4112-4120. Full text at ACS Publications
Chang SC, Fedeles BI, Wu J, Delaney JC, Li D, Zhao L, Christov PP, Yau E, Singh V, Jost M, Drennan CL, Marnett LJ, Rizzo CJ, Levine SS, Guengerich FP, Essigmann* JM. (2015). Next-generation sequencing reveals the biological significance of the N2, 3-ethenoguanine lesion in vivo. Nucleic Acids Research 43:5489-5500. Full text NAR
Jost M, Cracan V, Hubbard PA, Banerjee R, Drennan* CL. (2015). Visualization of a radical B12 enzyme with its G-protein chaperone. Proc. Natl. Acad. Sci. U.S.A. 112(8):2419-2424. Full text at PNAS
Gagnon DM, Brophy MB, Bowman SE, Stich TA, Drennan CL, Britt* RD, Nolan* EM. (2015). Manganese binding properties of human calprotectin under conditions of high and low calcium: X-ray crystallographic and advanced EPR spectroscopic analysis. Journal of the American Chemical Society 137(8):3004-3016. Full text at ACS Publications
Grell TA, Goldman PJ, Drennan* CL. (2014) SPASM and Twitch domains in S-adenosylmethione (SAM) radical enzyme structures. Journal of Biological Chemistry 290:3964-3971. Full text at JBC
Setser JW, Heemstra Jr JR, Walsh CT, Drennan* CL. (2014). Crystallographic evidence of drastic conformational changes in the active site of a flavin-dependent N-hydroxylase. Biochemistry 53(38):6063-6077. Full text at ACS Publications
Wei* Y, Funk MA, Rosado LA, Baek J, Drennan* CL, Stubbe* J. (2014). The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant. Proc. Natl. Acad. Sci. U.S.A. 111(36):E3756-E3765. Full text at PNAS
Liu DS, Nivón LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, Richardson D, Phipps WS, Ye A, Hell SW, Ellisman MW, Drennan CL, Baker D, Ting* AY. (2014) Computational design of a red fluorophore ligase for site-specific protein labeling in living cells. Proc. Natl. Acad. Sci. U.S.A. 111(43):E4551-E4559. Full text at PNAS
Funk MA, Judd ET, Marsh ENG, Elliott SJ, Drennan* CL. (2014) Structures of benzylsuccinate synthase elucidate roles of accessory subunits in glycyl radical enzyme activation and activity. Proc. Natl. Acad. Sci. U.S.A. 111(28):10161-10166. Full text at PNAS
Dowling DP, Bruender NA, Young AP, McCarty RM, Bandarian V, Drennan* CL. (2013). Radical SAM enzyme QueE defines a new minimal core fold and metal-dependent mechanism. Nature Chemical Biology 10:106-112. doi:10.1038/nchembio.1426
Goldman PJ, Grove TL, Booker SJ, Drennan* CL. (2013). X-ray analysis of butirosin biosynthetic enzyme BtrN redefines structural motifs for AdoMet radical chemistry. Proc. Natl. Acad. Sci. U.S.A. 110(40):15949-15954. Full text at PNAS
Goldman PJ, Grove TL, Sites LA, McLaughlin MI, Booker SJ, Drennan* CL. (2013). X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification. Proc. Natl. Acad. Sci. U.S.A. 110(21):8519-8524. Full text at PNAS
Li D, Fedeles BI, Shrivastav N, Delaney JC, Yang X, Wong C, Drennan CL, Essigmann* JM. (2013). Removal of N-alkyl modifications from N2-alkylguanine and N4-alkylcytosine by the adaptive response protein AlkB. Chemical Research in Toxicology. 26:1182-1187. Full text at ACS Publications
Sydor AM, Jost M, Ryan KS, Turo KE, Douglas CD, Drennan* CL, Zamble* DB. (2013) Metal binding properties of Escherichia coli YjiA, a member of the metal homeostasis-associated COG0523 family of GTPases. Biochemistry 52(10):1788-1801. Full text at ACS Publications
Kulik* HJ, Drennan CL. (2013) Substrate placement influences reactivity in non-heme Fe(II) halogenases and hydroxylases. J. Biol. Chem. 288:11233-11241. Full text at JBC
Minnihan EC, Ando N, Brignole EJ, Olshansky L, Chittuluru J, Asturias FJ, Drennan* CL, Nocera* DG, Stubbe* J. (2013) Generation of a stable, aminotyrosyl radical-induced α2β2 complex of Escherichia coli class Ia ribonucleotide reductase. Proc. Natl. Acad. Sci. U.S.A. 110(10):3835-3840 Full text at PNAS
Chang WC, Dey M, Liu P, Mansoorabadi SO, Moon S, Zhao ZK, Drennan CL, Liu* HW. (2013) Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction. Nature 496:114-118. Full text at Nature
Brignole‡ EJ, Ando‡ N, Zimanyi CM, Drennan* CL. (2012) The prototypic class Ia ribonucleotide reductase from Escherichia coli: Still surprising after all these years. Biochemical Society Transactions 40:523-530. Full text online
Li D, Delaney JC, Page CM, Yang X, Chen AS, Wong C, Drennan CL, Essigmann* JM. (2012) Exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB. J. Am. Chem. Soc. 134(21):8896-8901. Full text at ACS Publications
Ando N, Kung Y, Can M, Bender G, Ragsdale SW, Drennan CL. (2012) Transient B12-dependent methyltransferase complexes revealed by small-angle X-ray scattering. J. Am. Chem. Soc. 134(43):17945-17954. Full text at ACS Publications
Hamill MJ, Jost M, Wong C, Bene N, Drennan CL, Elliott* SJ. (2012) Electrochemical characterization of Escherichia coli adaptive response protein AidB. Int J Mol Sci 13:16899-16915. Full text
Bewley KD, Firer-Sherwood MA, Mock J-Y, Ando N, Drennan CL, Elliott* SJ. (2012) Mind the gap: Diversity and reactivity relationships among multihaem cytochromes of the MtrA/DmsE family. Biochem. Soc. Trans. 40:1268-1273. Full text at Bioch. Soc. Trans.
Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan* CL. (2012) An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes. Chemistry & Biology 19:855-865. Full text at Cell Press
Aye Y, Brignole EJ, Long MJC, Chittuluru J, Drennan CL, Asturias FJ, Stubbe* J. (2012) Clofarabine targets the large subunit (alpha) of human ribonucleotide reductase in live cells by assembly into persistent hexamers. Chemistry & Biology 19:799-805. Full text at Cell Press
Zimanyi CM, Ando N, Brignole EJ, Asturias FJ, Stubbe J, Drennan* CL. (2012) Tangled up in knots: Structures of inactivated forms of E. coli class Ia ribonucleotide reductase. Structure. 20(8):1374-1383. Full text at ScienceDirect
Dowling DP, Vey JL, Croft AK, Drennan* CL. (2012) Structural diversity in the AdoMet radical enzyme superfamily. Biochimica et Biophysica Acta (BBA): Proteins and Proteomics. 1824:1178-1195. Full text at ScienceDirect
Dowling DP, Croft AK, Drennan* CL. (2012) The radical use of Rossmann and TIM barrel architectures for controlling coenzyme B12 chemistry. Annual Review of Biophysics 41:403-427. Full text at Annual Reviews
Kung Y, Ando N, Doukov TI, Blasiak LC, Bender G, Seravalli J, Ragsdale SW, Drennan* CL. (2012) Visualizing molecular juggling within a B12-dependent methyltransferase complex. Nature 48:265-269. Full text at Nature
Setser JW, Lingaraju GM, Davis CA, Samson LD, Drennan* CL. (2012) Searching for DNA lesions: Structural evidence for lower- and higher-affinity DNA binding conformations of human alkyladenine DNA glycosylase. Biochemistry 51, 382-390. Full text at ACS Publications
Ando N, Brignole EJ, Zimanyi CM, Funk MA, Yokoyama K, Asturias FJ, Stubbe J, Drennan* CL. (2011) Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase. Proc. Natl. Acad. Sci. U.S.A. 108, 21046-21051. Full text at PNAS
Anderson WA, Amasino RM, Ares M Jr, Banerjee U, Bartel B, Corces VG, Drennan CL, Elgin SC, Epstein IR, Fanning E, Guillette LJ Jr, Handelsman J, Hatfull GF, Hoy RR, Kelley D, Leinwand LA, Losick R, Lu Y, Lynn DG, Neuhauser C, O’Dowd DK, Olivera T, Pevzner P, Richards-Kortum RR, Rine J, Sah RL, Strobel SA, Walker GC, Walt DR, Warner IM, Wessler S, Willard HF, Zare RN. (2011) Competencies: A cure for pre-med curriculum. Science 334:760-761. Full text at Science
Hamill‡ MJ, Jost‡ M, Wong C, Elliott* SJ, Drennan* CL. (2011) Flavin-induced oligomerization in Escherichia coli adaptive response protein AidB. Biochemistry 50:10159-10169. Full text at ACS Publications
Firer-Sherwood‡ MA, Ando‡ N, Drennan CL, Elliott* SJ. (2011) Solution-based structural analysis of the decaheme cytochrome, MtrA, by small-angle X-ray scattering and analytical ultracentrifugation. J. Phys. Chem. B 115:11208-11214. Full text at ACS Publications
Anderson WA, Banerjee U, Drennan CL, Elgin SC, Epstein IR, Handelsman J, Hatfull GF, Losick* R, O’Dowd* DK, Olivera BM, Strobel SA, Walker GC, Warner IM. (2011) Changing the culture of science education at research universities. Science 331:152-153. Full text at Science
Yun D, Dey M, Higgins LJ, Yan F, Liu H-W, Drennan* CL. (2011) Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis. J. Am. Chem. Soc. 131:11262-11269. Full text at ACS Publications
Lingaraju GM, Davis CA, Setser JW, Samson* LD, Drennan* CL. (2011) Structural basis for the inhibition of human alkyladenine DNA glycosylase (AAG) by 3,N4-ethenocytosine–containing DNA. J. Biol. Chem. 286:13205-13213. Full text at JBC
Vey JL, Drennan* CL. (2011) Structural insights into radical generation by the radical SAM superfamily. Chem. Rev. 111:2487-2506. Full text at ACS Publications
Kung Y, Drennan* CL. (2011) A role for nickel-iron cofactors in biological carbon monoxide and carbon dioxide utilization. Curr. Opin. Biol. Chem. 15:276-283. Full text at ScienceDirect
Li D, Delaney J, Page C, Chen A, Wong C, Drennan CL, Essigmann* JM. (2010) Repair of DNA alkylation damage by the Escherichia coli adaptive response protein AlkB as studied by ESI-TOF mass spectrometry. J. Nucleic Acids 2010:369434. doi: 10.4061/2010/369434.
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Phillips CM, Schreiter ER, Stultz CM, Drennan* CL. (2010) Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli. Biochemistry. 49:7830-7838. Full text at ACS Publications
Phillips CM, Stultz CM, and Drennan* CL. (2010) Searching for the nik operon: How a ligand-responsive transcription factor hunts for its DNA binding site. Biochemistry. 49:7757-7763. Full text
Taylor* EV, Fortune JA, Drennan CL. (2010) A research-inspired laboratory sequence investigating acquired drug resistance. Biochem. Mol. Biol. Educ. 38:247-252. Full text
Taylor* EV, Mitchell R, Drennan CL. (2009) Creating an interdisciplinary introductory chemistry course without time-intensive curriculum changes. ACS Chem. Biol. 4:979-982. Full text at ACS Publications
Kulik* HJ, Blasiak LC, Marzari N, Drennan CL. (2009) First-principles study of non-heme Fe(II) halogenase SyrB2 reactivity. J. Am. Chem. Soc. 131:14426-14433. Full text at ACS Publications
Drennan* CL, Jarrett* JT. (2009) From single molecules to whole organisms: The evolving field of mechanistic enzymology. Curr. Opin. Chem. Biol. 13:433-435. Full text
Kung Y, Doukov TI, Seravalli J, Ragsdale SW, Drennan* CL. (2009) Crystallographic snapshots of cyanide- and water-bound C-Clusters from bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Biochemistry. 48(31):7432-7440. Full text at ACS Publications
Phillips CM, Nerenberg PS, Drennan CL, Stultz* CM. (2009) Physical basis of metal-binding specificity in Escherichia coli NikR. J. Am. Chem. Soc. 131(29):10220-10228. Full text at ACS Publications
Ryan KS, Drennan* CL. (2009) Divergent pathways in the biosynthesis of bisindole natural products. Chem. Biol. 16:351-364. Full article
Wong C, Fujimori DG, Walsh CT, Drennan* CL. (2009) Structural analysis of an open active site conformation of nonheme iron halogenase CytC3. J. Am. Chem. Soc. 131:4872-4879. Full text at ACS Publications
Blasiak, LC and Drennan* CL. (2009) Structural perspective on enzymatic halogenation. Acc. Chem. Res. 42(1):147-155. Full text at ACS Publications
Ryan KS, Chakraborty S, Howard-Jones AR, Walsh CT, Ballou DP, Drennan* CL. (2008) The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction. Biochemistry. 47:13506-13513. Full text at ACS Publications
Vey JL, Yang J, Li M, Broderick WE, Broderick JB, Drennan* CL. (2008) Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme. Proc. Natl. Acad. Sci. U.S.A. 105(42):16137-16141. Full article
Phillips CM, Drennan CL. (2008) Nickel regulatory transcription factor, NikR. Handbook of Metalloproteins, Wiley.
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Hernandez HH, Jaquez OA, Hamill MJ, Elliott SJ, Drennan* CL. (2008) Thioredoxin reductase from Thermoplasma acidophilum: A new twist on redox regulation. Biochemistry. 47(37):9728-9737. Full text at ACS Publications
Hamill MJ, Chobot SE, Hernandez HH, Drennan CL, Elliott* SJ. (2008) Direct electrochemical analyses of a thermophilic thioredoxin reductase: Interplay between conformational change and redox chemistry. Biochemistry. 47(37):9738-9746. Full text at ACS Publications
Ryan KS, Balibar CJ, Turo KE, Walsh CT, Drennan* CL. (2008) The violacein biosynthetic enzyme VioE shares a fold with lipoprotein transporter proteins. J. Biol. Chem. 283(10):6467-6475. Full article
Doukov TI, Blasiak LC, Seravalli J, Ragsdale SW, Drennan* CL. (2008) Xenon in and at the end of the tunnel of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Biochemistry 47(11):3474-3483. Full text at ACS Publications
Phillips CM, Schreiter ER, Guo Y, Wang SC, Zamble DB, Drennan* CL. (2008) Structural basis of the metal specificity for nickel regulatory protein NikR. Biochemistry 47(7):1938-1946. Full text at ACS Publications
Hubbard PA, Padovani D, Labunska T, Mahlstedt SA, Banerjee R, Drennan* CL. (2007) Crystal structure and nutagenesis of the netallochaperone MeaB. J. Biol. Chem. 282(43):31308-31316. Full article
Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan* CL. (2007) Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC. Proc. Natl. Acad. Sci. U.S.A. 104(39):15311-15316. Full article
Schreiter* ER, Drennan CL. (2007) Ribbon-helix-helix transcription factors: Variations on a theme. Nat. Rev. Microbiol. 5(9):710-720. Full article
Chobot SE, Hernandez HH, Drennan CL, Elliott* SJ. (2007) Direct electrochemical characterization of archeael thioredoxins. Angew. Chem. Int. Ed. Engl. 46(22):4145-4147. Full article
Doukov‡ TI, Hemmi‡ H, Drennan CL, Ragsdale* SW. (2007) Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer: Role of an active site asparagine residue in activation of methyl transfer by methyltransferases. J. Biol. Chem. 282(9):6609-6618. Full article
Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh* CT. (2007) Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases. Biochemistry. 46(5):1284-1292. Full article
Frick LE, Delaney JC, Wong C, Drennan CL, Essigmann* JM. (2007) Alleviation of 1,N6-ethanoadenine genotoxicity by the Escherichia coli adaptive response protein AlkB. Proc. Natl. Acad. Sci. U.S.A. 104(3):755-760. Full article
Blasiak LC, Vaillancourt FH, Walsh CT, Drennan* CL. (2006) Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis. Nature. 440:368-371. Full article
Schreiter ER, Wang SC, Zamble DB, Drennan* CL. (2006) NikR-operator complex structure and the mechanism of repressor activation by metal ions. Proc. Natl. Acad. Sci. U.S.A. 103(37):13676-13681. Full article
Delaney JC, Smeester L, Wong C, Frick LE, Taghizadeh L, Wishnok JS, Drennan CL, Samson LD, Essigmann* JM. (2005) AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo. Nat. Struct. Mol. Biol. 12(10):855-860. Full article
Higgins LJ, Yan F, Liu P, Liu H-W, Drennan* CL. (2005) Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme. Nature 437:838-844. Full article
Berkovitch F, Behshad E, Tang KH, Enns EA, Frey PA, Drennan* CL. (2004) A locking mechanism preventing radical damage in the absence of substrate, as revealed by the X-ray structure of lysine-5,6-aminomutase. Proc. Natl. Acad. Sci. U.S.A. 101(45):15870-15875. Full article
Midelfort KS, Hernandez HH, Lippow SM, Tidor* B, Drennan* CL, Wittrup* KD. (2004) Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J. Mol. Biol. 343(3):685-701. Full article
Tirupati B, Vey JL, Drennan CL, Bollinger* Jr. JM. (2004) Kinetic and structural characterization of Slr0077/SufS, the essential cysteine desulfurase from Synechocystis sp. PCC 6803. Biochemistry 43:12210-12219. Full article
Nicolet Y, Drennan* CL. (2004) AdoMet radical proteins—from structure to evolution: Alignment of divergent protein sequences reveals strong secondary structure element conservation. Nucleic Acids Res. 32:4015-4025. Full article
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Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan* CL. (2004) Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme. Science 303(5654):76-79. Full article
Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan* CL. (2003) Crystal structure of the nickel-responsive transcription factor NikR. Nat. Struct. Biol. 10(10):794-799. Full article
Drennan* CL, Peters* JW. (2003) Surprising cofactors in metalloenzymes. Curr. Opin. Struct. Biol. 13(2):220-226. Full article
Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan* CL. (2002) A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Science 298(5593):567-572. Full article
Sintchak MD, Arjara G, Kellogg BA, Stubbe J, Drennan* CL. (2002) The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer. Nat. Struct. Biol. 9(4):293-300. Full article
Drennan* CL, Heo J, Sintchak MD, Schreiter E, Ludden PW. (2001) Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase. Proc. Natl. Acad. Sci. U.S.A. 98(21):11973-11978. Full article
Marsh* EN, Drennan CL. (2001) Adenosylcobalamin-dependent isomerases: New insights into structure and mechanism. Curr. Opin. Chem. Biol. 5(5):499-505. Full article