Updated Apr 2025
Westmoreland, D.E., Feliciano‡, P.R., Kang, G., Cui, C., Kim, A., Stubbe, J., Nocera, D.G., and Drennan*, C.L. (2024) 2.6-Å Resolution Cryo-EM Structure of a Class Ia Ribonucleotide Reductase Trapped with Mechanism-based Inhibitor N3CDP, Proc. Natl. Acad. Sci. U.S.A. 121 (45) e2417157121, doi.org/10.1073/pnas.2417157121 (2024). PMID: 39475643; PMCID: PMC11551348. PDBID: 9DB2, EMPIAR: 12249 , EMDB: EMD-46711.
Biester, A., Grahame, D.A., Drennan*, C.L. (2024) Capturing a Methanogenic Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase Complex via Cryogenic Electron Microscopy Proc. Natl. Acad. Sci. 121 (41) e2410995121, doi.org/10.1073/pnas.2410995121 (2024). PMID: 39361653; PMCID: PMC11474084. PDBID: 9C0Q, 9C0R, 9C0S, 9C0T. EMPIAR: 12108, 12124. EMDB: EMD-45089, EMD-45090, EMD-45091, EMD-45092.
Adak, S., Ye, N., Calderone, L.A. et al. A single diiron enzyme catalyses the oxidative rearrangement of tryptophan to indole nitrile. Nat. Chem. 16, 1989–1998 (2024). doi.org/10.1038/s41557-024-01603-z PMID: 37577561; PBD: 8TWN , 8TWT, 8TWW
Funk, M.A., Zimanyi, C.M., Andree, G.A., Hamilos, A.E., Drennan, C.L. (2024) How ATP and dATP Act as Molecular Switches to Regulate Enzymatic Activity in the Prototypical Bacterial Class Ia Ribonucleotide Reductase, Biochemistry Special Issue in Honor of Christopher T. Walsh, doi: 10.1021/acs.biochem.4c00329. PMID: 39164005. PMCID: PMC11447812 ; PDBID: 8VHN, 8VHO, 8VHP, 8VHQ, 8VHR, 8VHU
Coco, L.B., Toci, E.M., Chen, P.T-Y., Drennan*, C.L., and Freel Meyers*, C.L. (2024) Potent Inhibition of E. coli DXP Synthase by a gem-Diaryl Bisubstrate Analog, ACS Infect. Dis., 1312-1326 accepted. https://doi.org/10.1021/acsinfecdis.3c00734. PMCID: PMC11019550. PMID: 38513073 PDB: 6OUV , 6OUW
Vasquez, S., Marquez, M.D., Brignole, E.J., Vo, A., Kong, S. Park, C., Perlstein*, D.L., and Drennan*, C.L. (2023) Structural and Biochemical Investigations of a HEAT-repeat Protein Involved in the Cytosolic Iron-Sulfur Cluster Assembly Pathway, Commun. Biol. 6, 1276. doi:10.1038/s42003-023-05579-3. PMID: 38110506 PMCID: PMC10728100
Vaccaro, F.A., Faber, D.A., Andree, G.A., Born, D.A., Kang, G., Fonseca, D.R., Jost, M., and Drennan*, C.L. (2023) Structural Insight into G-protein Chaperone-mediated Maturation of a Bacterial Adenosylcobalamin-dependent Mutase, J. Biol. Chem. 299, 105109. doi: https://doi.org/10.1016/j.jbc.2023.105109 PMCID: PMC10481361. PMID: 37517695 PDB: 8SSL, 8STA EMDB: EMD-40751, EMD-40758
Andorfer, M.C., King-Roberts, D.T., Imrich, C.N., Brotheridge, B.G., and Drennan*, C.L. (2023) Development of an in vitro Method for Activation of X-succinate Synthases for Fumarate Hydroalkylation, iScience 26(6), 106902. doi: 10.1016/j.isci.2023.106902. PMCID: PMC10239695. PMID: 37283811
Cui, C., Song, D.Y., Drennan, C.L., Stubbe*, J., and Nocera*, D.G. (2023) Radical Transport Facilitated by a Proton Transfer Network at the Subunit Interface of Ribonucleotide Reductase, J. Am. Chem. Soc. ASAP February 22, 2023 doi: 10.1021/jacs.2c11483 PMCID: PMC10561588. PMID: 36812162
Vaccaro, F.A., Born, D.A, and Drennan*, C.L. (2023) Structure of Metallochaperone in Complex with the Cobalamin-binding Domain of its Target Mutase Provides Insight into Cofactor Delivery, Proc. Natl. Acad. Sci. doi: 10.1073/pnas.2214085120. PMCID: PMC9974510.PMID: 36787360 PDB: 8DPB
Levitz, T.S. and Drennan*, C.L. (2022) Starting a New Chapter on Class Ia Ribonucleotide Reductases, Curr. Opin. Struct. Biol. 77,102489. PMID: 36272229 doi: 10.1016/j.sbi.2022.102489.
Biester, A., Marcano-Delgado, A.N., Drennan*, C.L. (2022) Structural Insights into Microbial One-Carbon Metabolic Enzymes Ni−Fe−S-dependent Carbon Monoxide Dehydrogenases and Acetyl-CoA Synthases, Biochemistry 61(24), 2797-2805. doi: 10.1021/acs.biochem.2c00425. PMCID: PMC9782325. PMID: 36137563
Sankari, S., Babu, V.M.P, Bian, K., Alhazmi, A., Andorfer, M.C., Avalos, D.M., Smith, T.A., Yoon, K., Drennan, C.L., Yaffe M.B., Lourido, S., and Walker*, G.C. (2022) Haem-sequestering Plant Peptide Drives Iron Uptake in Symbiotic Bacteria, Nat. Microbiol. 7(9), 1453-1465. doi: 10.1038/s41564-022-01192-y. PMCID: PMC9420810. PMID: 35953657 | Highlighted in MIT News
Levitz, T.S., Weckener, M. Fong, I., Naismith, J.H., Drennan, C.L., Brignole, E.J., Clare, D.K., Darrow*, M.C. (2022) Approaches to Using the chameleon: Robust, Automated, Fast-plunge CryoEM Specimen Preparation, Front. Mol. Biosci. 9, 903148. doi: 10.3389/fmolb.2022.903148. PMCID: PMC9260054. PMID: 35813832
Vaccaro, F.A. and Drennan*, C.L (2022) The Role of Nucleoside Triphosphate Hydrolase Metallochaperones in Making Metalloenzymes, Metallomics 14(6), mfac030. In honor of Deborah Zamble. doi:10.1093/mtomcs/mfac030. PMCID: PMC9164220
Ulrich, E.C. and Drennan*, C.L. (2022) The Atypical Cobalamin-Dependent S-Adenosyl-L-Methionine Nonradical Methylase TsrM and its Radical Counterparts, J. Am. Chem. Soc. 144(13), 5673-5684. https://doi.org/10.1021/jacs.1c12064. PMCID: PMC8992657. PMID: 35344653
Biester, A., Dementin, S., and Drennan*, C.L. (2022) Visualizing the Gas Channels of a Monofunctional Carbon Monoxide Dehydrogenase, J. Inorg. Biochem. 230, 111774. Special issue in honor of Dick Holm. doi:10.1016/j.jinorgbio.2022.111774. PMCID: PMC9093221. PMID: 35278753 PDB: 7TSJ
Ohmer, C.J., Dasgupta, M., Patwardhan, A., Bogacz, I., Kaminsky, C., Doyle, M.D., Chen, P. Y.-T., Keable, S.M., Makita, H., Simon, P.S., Massad, R., Fransson, T., Chatterjee, R., Bhowmick, A., Paley, D.W., Moriarty, N.W., Brewster, A.S., Gee, L.B., Alonso-Mori, R., Moss, F., Fuller, F.D., Batyuk, A., Sauter, N.K., Bergmann, U., Drennan, C.L., Yachandra, V.K., Yano*, J., Kern*, J.F., Ragsdale*, S.W. (2022) XFEL Serial Crystallography Reveals the Room Temperature Structure of Methyl-Coenzyme M Reductase, J. Inorg. Biochem. 230, 111768. Special issue in honor of Dick Holm. doi: 10.1016/j.jinorgbio.2022.111768. PMID: 35202981 PMCID: PMC8930625 PDB: 7SXM, 7SUC
Narasimhan*, J., Letinski, S., Jung, S., Gerasyuto, A., Wang, J., Arnold, M., Chen, G., Hedrick, J., Dumble, M., Ravichandran, K., Levitz, T.L., Chang, C., Drennan, C.L., Stubbe*, J., Karp, G., and Branstrom*, A. (2022) Ribonucleotide Reductase, A Novel Drug Target for Gonorrhea, eLife 11:e67447. doi:10.7554/eLife.67447. PMCID: PMC886584. PMID: 35137690.
Bridwell-Rabb*, J., Li, B., Drennan, C.L. (2022) Cobalamin-dependent Radical S-adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions, ACS Bio. & Med. Chem. 2(3), 173-186. https://doi.org/10.1021/acsbiomedchemau.1c00051. PMCID: PMC9204698
Levitz, T.S., Brignole, E.J., Fong, I., Darrow*, M.C., and Drennan*, C.L. (2021) Effects of chameleon Dispense-to-plunge Speed on Particle Concentration, Complex Formation, and Final Resolution: A Case Study Using the Neisseria gonorrhoeae Ribonucleotide Reductase Inactive Complex, J. Struct. Biol. 214, 107825. doi: 10.1016/j.jsb.2021.107825. PMCID: PMC8994553. PMID: 34906669 PDB: 7MDI, EMD-23773
Andorfer, M.C., Backman, L.R.F., Li, P.L., Ulrich, E.C., and Drennan*, C.L. (2021) Rescuing Activity of Oxygen-damaged Pyruvate Formate-Lyase By a Spare Part Protein, J. Biol. Chem. 297(6), 101423. doi: 10.1016/j.jbc.2021.101423. PMCID: PMC8683613. PMID: 34801558
Feliciano, P.R., Carroll, K.S., and Drennan*, C.L. (2021) Crystal Structure of the [4Fe-4S] Cluster-containing Adenosine-5′-Phosphosulfate Reductase from Mycobacterium tuberculosis, ACS Omega 6(21), 13756-13765. ao1c01043. doi: 10.1021/acsomega.1c01043. PMCID: PMC8173546 PDB: 7LHR, 7LHS, 7LHU
Andorfer*, M.C. and Drennan, C.L. (2021) Fixing Nature’s Carbon Inefficiencies, Joule 5, 765–767. doi: 10.1016/j.joule.2021.03.025
Dawson ‡, C.D., Irwin‡, S.M., Backman, L.R.F., Le, C., Wang, J.X., Vennelakanti, V., Yang, Z., Kulik*, H.J., Drennan*, C.L., and Balskus*, E.P. (2021) Molecular Basis of C–S Bond Cleavage in the Glycyl Radical Enzyme Isethionate Sulfite-Lyase, Cell Chem. Biol. 28(9), 1333-1346. doi: 10.1016/j.chembiol.2021.03.001. PMCID: PMC8473560. PMID: 33773110 PDB: 7KQ3, 7KQ4
Knox, H.L., Chen, P. Y.T., Blaszczyk, A.J., Mukherjee, A., Grove, T.L., Schwalm, E.L., Wang, B., Drennan*, C.L., and Booker*, S.J. (2021) Structural Basis for Non-Radical Catalysis by TsrM, a Radical SAM Methylase, Nat. Chem. Biol. 17(4), 485-491. doi: 10.1038/s41589-020-00717-y PMCID: PMC7990684. PMID: 33462497 PDB: 6WTE, 6WTF
Jonnalagadda‡, R., Del Rio Flores‡, A., Cai, W., Mehmood, R., Narayanamoorthy, M., Ren, C., Zaragoza, J.P.T., Kulik*, H.J., Zhang*, W., and Drennan*, C.L. (2021) Biochemical and Crystallographic Investigations into Isonitrile Formation by a Nonheme Iron-Dependent Oxidase/Decarboxylase, J. Biol. Chem. 296, 100231. doi: 10.1074/jbc.RA120.015932. PMCID: PMC7949033. PMID: 33361191 PDB: 6XN6, 6XO3, 6XOJ, 6XPA
Cui, C., Greene*, B.L., Kang, G., Drennan, C.L., Stubbe*, J., Nocera*, D.G. (2020) Gated Proton Release during Radical Transfer at the Subunit Interface of Ribonucleotide Reductase, J. Am. Chem. Soc. 143, 176-183. doi: 10.1021/jacs.0c07879. PMCID: PMC7904477
Cohen, S.E., Brignole, E.J., Wittenborn, E.C., Can, M., Thompson, S., Ragsdale, S.W., and Drennan*, C.L. (2021) Negative Stain Electron Microscopy Reveals Dramatic Structural Rearrangements in Ni-Fe-S Dependent Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase, Structure 29, 43-49. doi: 10.1016/j.str.2020.08.011. PMCID: PMC7796957. PMID: 32937101
Cohen, S.E., Can, M., Wittenborn, E.C., Hendrickson, R.A., Ragsdale, S.W., Drennan*, C.L. (2020) Crystallographic Characterization of the Carbonylated A-cluster in Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase, ACS Catalysis 10(17), 9741-9746. doi: 10.1021/acscatal.0c03033. PMCID: PMC7819276. PMID: 33495716 PDB: 6X5K
Reinhardt, C., Li, P., Kang, G., Stubbe, J., Drennan, C.L., Hammes-Schiffer*, S. (2020) Conformational Motions and Water Networks at the α/β Interface in E. coli Ribonucleotide Reductase. J. Am. Chem. Soc. 142(32), 13768-13778. doi: 10.1021/jacs.0c04325. PMCID: PMC7594210
Greene, B.L., Kang, G., Cui, C., Bennati, M., Nocera, D.G., Drennan, C.L., and Stubbe, J. (2020) Ribonucleotide Reductases (RNRs): Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets, Ann. Rev. Biochem. 89, 45-75. doi: 10.1146/annurev-biochem-013118-111843. PMCID: PMC7316142
Rizzolo, K., Weitz, A.C., Cohen, S.E., Drennan, C.L, Hendrich, M.P., and Elliott*, S.J. (2020) A Stable Ferryl Porphyrin at the Active Site of Y463M BthA, J. Am. Chem. Soc. 142(28), 11978-11982. doi: 10.1021/jacs.0c04023. PMCID: PMC8667324
Wittenborn, E.C., Guendon, C., Merrouch, M., Benvenuti, M., Fourmond, V., Léger, C., Drennan*, C.L., and Dementin*, S. (2020) The Solvent-Exposed Fe-S D-cluster Contributes to Oxygen-Resistance in D. vulgaris Ni-Fe Carbon Monoxide Dehydrogenase, ACS Catalysis 10(13), 7328-7335. doi: 10.1021/acscatal.0c00934. PMCID: PMC7343238 PDB: 6VWY, 6VWZ, 6VX0, 6VX1
Kang, G., Taguchi, A.T., Stubbe*, J., and Drennan*, C.L. (2020) Structure of a Trapped Radical Transfer Pathway within a Ribonucleotide Reductase Holocomplex, Science 368(6489), 424-427. DOI: 10.1126/science.aba6794. PMCID: PMC7774503. PMID: 32217749 PDB: 6W4X | EMD-21540
Bollenbach, M., Ortega, M. Orman, M. Drennan, C.L., and Balskus*, E.P. (2020) Discovery of a Cyclic Choline Analog that Inhibits Anaerobic Choline Metabolism by Human Gut Bacteria, ACS Med. Chem. Let. 11(10), 1980-1985. doi: 10.1021/acsmedchemlett.0c00005. PMCID: PMC7549264. PMID: 33062182 PDB: 6VUE
Backman‡, L.R.F., Huang‡, Y.Y., Andorfer, M.C., Gold, B., Raines, R.T., Balskus*, E.P., and Drennan*, C.L. (2020) Molecular Basis for Catabolism of the Abundant Metabolite Trans-4-hydroxy-L-Proline by a Microbial Glycyl Radical Enzyme, eLife 9, e51420. doi: 10.7554/eLife.51420. PMCID: PMC7077986. PMID: 32180548 PDB: 6VXC, 6VXE
Highlighted by MIT News, “Bacterial enzyme could become a new target for antibiotics” by Anne Trafton
Feliciano*, P.R. and Drennan*, C.L. (2019) Structural and Biochemical Investigations of the [4Fe4S] Cluster-containing Fumarate Hydratase from Leishmania major, Biochemistry 58(49), 5011-5021. doi: 10.1021/acs.biochem.9b00923. PMCID: PMC7065722 PDB: 6UNZ, 6UO0, 6UOI, 6UOJ, 6UP9, 6UPM, 6UPO, 6UQ8, 6UQ9, 6UQB, 6UQL, 6UQM, 6UQN
Wittenborn, E.C., Cohen, S.E., Merrouch, M., Léger, C., Fourmond, V., Dementin*, S., and Drennan*, C.L. (2019) Structural Insight into Metallocofactor Maturation in Carbon Monoxide Dehydrogenase, J. Biol. Chem. 294(35), 13017-13026. doi: 10.1074/jbc.RA119.009610. PMCID: PMC6721931. PDB: 6ONC, 6OND, 6ONS
Faculty of 1000 Recommended.
Chen, P.Y.T., DeColli, A.A., Freel Meyers*, C.L., and Drennan*, C.L. (2019) X-ray Crystallography–based Structural Elucidation of Enzyme-bound Intermediates Along the 1–Deoxydxylulose 5-phosphate Synthase Reaction Coordinate, J. Biol. Chem. 294(33), 12405-12414. doi: 10.1074/jbc.RA119.009321. PMCID: PMC6699841 PDB: 6OUV, 6OUW
Bowman, S.E.J, Backman, L.R.F., Bjork, R.E., Andorfer, M.C., Yori, S., Caruso, A., Stultz, C.M., and Drennan*, C.L., (2019) Solution Structure and Biochemical Characterization of a Spare Part Protein That Restores Activity to an Oxygen-damaged Glycyl Radical Enzyme, J. Biol. Inorg. Chem. Special Issue in Honor of Joan Broderick 24(6), 817-829. doi: 10.1007/s00775-019-01681-2. PMCID: PMC6754787. PMID: 31250200 PDB: 6OWR
Highlighted by MIT Biology: “Bacteria use ‘spare part’ proteins to repair damage and survive inhospitable conditions” by Saime Sidik.
Bian, K., Lenz, S.A.P., Tang, Q., Chen, F., Qi, R., Jost, M., Drennan, C.L., Essigmann, J.M., Wetmore, S.D., Li*, D. (2019) DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB Oxidize 5-Methylcytosine to 5-Hydroxymethylcytosine, 5-Formylcytosine and 5-Carboxylcytosine in vitro, Nucleic Acids Res. 47(11), 5522-5529. doi: 10.1093/nar/gkz395. PMCID: PMC6582317
Rizzolo, K., Cohen, S.E., Weitz, A.C., López Muñoz, M.M., Hendrich, M.P., Drennan, C.L., and Elliott*, S.J. (2019) A Widely Distributed Diheme Enzyme from Burkholderia that Displays an Atypically Stable bis–Fe(IV) State, Nature Comm. 10, 1101. doi: 10.1038/s41467-019-09020-4. PMCID: PMC6405878 PDB: 6NXO
Feliciano, P.R., Drennan*, C.L., and Nonato*, M.C. (2019) Crystal Structures of Fumarate Hydratases from Leishmania major in a Complex with Inhibitor 2-Thiomalate, ACS Chem. Biol. 14(2), 266-275. doi: 10.1021/acschembio.8b00972. PMCID: PMC6380369 PDB: 6MSN , 6MSO
Chen‡, P.Y-T., Li‡, B., Drennan*, C.L., and Elliott*, S.J. (2019) A Reverse TCA Cycle 2-Oxoacid:ferredoxin Oxidoreductase that Makes C-C Bonds from CO₂, Joule 3(2), 595-611. doi: 10.1016/j.joule.2018.12.006. PMCID: PMC6508887. PMID: 31080943 PDB: 6N2N , 6N2O
Orman, M., Bodea, S., Funk, M.A., Martinez-del Campo, A., Bollenbach, M., Drennan, C.L., and Balskus*, E.P. (2019) Structure-Guided Identification of a Small Molecule that Inhibits Anaerobic Choline Metabolism by Human Gut Bacteria. J. Am. Chem. Soc. 141, 33-37. doi: 10.1021/jacs.8b04883. PMCID: PMC6475491 PDB: 6ND3
Grell, T.A.J., Bell, B.N., Nguyen, C., Dowling, D.P., Bruender, N.A., Bandarian, V., and Drennan*, C.L. (2019) Crystal Structure of AdoMet Radical Enzyme 7-Carboxy-7-Deazaguanine Synthase from Escherichia coli Suggests How Modifications Near [4Fe-4S] Cluster Engender Flavodoxin Specificity, Protein Science 28, 202-215. doi: 10.1002/pro.3529. PMCID: PMC6295903. PMID: 30341796. PDB: 6NHL
Grell, T.A.J., Kincannon, W.M., Bruender, N.A., Blaesi, E.J., Krebs*, C., Bandarian*, V., and Drennan*, C.L. (2018) Structural and Spectroscopic Analysis of Sporulation Killing Factor Biosynthetic Enzyme SkfB, a bacterial AdoMet Radical Sactisynthase, J. Biol. Chem. 293(45), 17349-17361. doi: 10.1074/jbc.RA118.005369. PMCID: PMC6231123. PMID: 30217813
Wittenborn, E.C., Merrouch, M., Ueda, C., Fradale, L., Léger, C., Fourmond, V., Pandelia, M-E., Dementin*, S., and Drennan*, C.L. (2018) Redox-Dependent Rearrangements of the NiFeS Cluster of Carbon Monoxide Dehydrogenase, eLife 7, e39451. doi: 10.7554/eLife.39451. PMCID: PMC6168284. PMID: 30277213
Highlighted by MIT News and APS highlights: “An Unexpected Enzyme Structure” by Anne Trafton; and by Chemical and Engineering News: “Metal Secrets of CO2-converting Enzyme Revealed” by Cici Zhang; Faculty of 1000 Recommended
Ryan, K.S., and Drennan, C.L. (2018) Metalloenzymes in Natural Product Biosynthetic Pathways, Nat. Prod. Rep. 35(7), 612-614. doi: 10.1039/C8NP90023J
Harris, N.C., Born, D.A., Cai, W., Huang, Y., Martin, J., Khalaf, R., Drennan, C.L., and Zhang*, W. (2018) Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase, Angew. Chemie Int. Ed. 57(31), 9707-9710. doi: 10.1002/anie.201804307. PMCID: PMC6191297. PMID: 29906336
Grell‡, T.A.J., Young‡, A.P., Drennan*, C.L., and Bandarian*, V. (2018) Biochemical and Structural Characterization of a Schiff Base in the Radical-Mediated Biosynthesis of 4-Demethylwyosine by TYW1, J. Am. Chem. Soc. 140(22), 6842-6852. doi: 10.1021/jacs.8b01493. PMCID: PMC5994729. PMID: 29792696
Faculty of 1000 Recommended
Nakashige, T.G., Bowman, S.E.J., Zygiel, E.M., Drennan*, C.L., and Nolan*, E.M. (2018) Biophysical Examination of the Calcium-Modulated Nickel-Binding Properties of Human Calprotectin Reveals Conformational Change in the EF-Hand Domains and His3Asp Site, Biochemistry 57(28), 4155-4164. doi: 10.1021/acs.biochem.8b00415. PMCID: PMC6050108
Chen‡, P.Y.-T., Funk‡, M.A., Brignole, E.J., and Drennan*, C.L. (2018) Disruption of an Oligomeric Interface Prevents Allosteric Inhibition of Escherichia coli class Ia Ribonucleotide Reductase, J. Biol. Chem. 293(26), 10404-10412. doi: 10.1074/jbc.RA118.002569. PMCID: PMC6028975. PMID: 29700111
Chen PY-T, Aman H, Can M, Ragsdale SW, Drennan* CL. (2018) Binding site for coenzyme A revealed in the structure of pyruvate:ferredoxin oxidoreducatase from Moorella thermoacetica. Proc. Natl. Acad. Sci. U.S.A. Full text at PNAS
Brignole‡ EJ, Tsai‡ KL, Chittuluru J, Li H, Aye Y, Penczek PA, Stubbe* J, Drennan* CL, Asturias* F. (2018) 3.3-Å resolution cryo-EM structure of human ribonucleotide reductase with substrate and allosteric regulators bound. eLife 7:e31502. Full text at eLife
Bridwell-Rabb* J, Grell TAJ, Drennan* CL. (2018) A rich man, poor man story of S-adenosylmethionine and cobalamin revisited. Ann. Rev. of Biochem. 87:555-584. DOI: 10.1146/annurev-biochem-062917-012500
Born‡ DA, Ulrich‡ EC, Ju KS, Peck SC, van der Donk* WA, Drennan* CL. (2017) Structural basis for methylphosphonate biosynthesis. Science 358(6368):1336-1339. Full text at Science
Backman LRF, Funk MA, Dawson CD, Drennan* CL. (2017) New tricks for the glycyl radical enzyme family. Critical Reviews In Biochemistry & Molecular Biology 52(6):674-695. Full text
Shisler K, Hutcheson R, Horitani M, Duschene K, Crain A, Byer A, Shepard E, Rasmussen A, Yang J, Broderick W, Vey J, Drennan CL, Hoffman B, Broderick* J. (2017) Monovalent cation activation of the radical SAM enzyme pyruvate formate-lyase activating enzyme. J. Am. Chem. Soc. 139(34):11803-11813. Full text at J. Am. Chem. Soc.
Bridwell-Rabb‡ J, Zhong‡ A, Sun HG, Drennan* CL, Liu* H-w. (2017) A B12-dependent radical SAM enzyme involved in oxetanocin-A biosynthesis. Nature 544:322-326. Full text at Nature
Kung* Y, Drennan CL. (2017) One-carbon chemistry of nickel-containing carbon monoxide dehydrogenase and acetyl-CoA synthase. In The Biological Chemistry of Nickel, D. Zamble, M. Rowinska-Zyrek, H. Kozlowski, eds., Royal Society of Chemistry, London, pp. 121-148. DOI: 10.1039/9781788010580-00121
Cory SA, Van Vranken JG, Brignole EJ, Patra S, Winge DR, Drennan CL, Rutter J, Barondeau* DP. (2017) Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions, Proc. Natl. Acad. Sci. U.S.A. . Full text at PNAS
Lin Q, Parker MJ, Taguchi AT, Ravichandran K, Kim A, Kang G, Drennan* CL, Stubbe* J. (2017) Glutamate 52-β at the α/β subunit interface of E. coli class Ia ribonucleotide reductase is essential for conformational gating of radical transfer. J. Biol. Chem. 292:9229-9239. Full text at J. Biol. Chem.
Nakashige‡ TG, Zygiel‡ EM, Drennan* CL, Nolan* EM. (2017) Nickel sequestration by the host-defense protein human calprotectin. J. Am. Chem. Soc. 139:8828-8836. Full text at JACS
Padmanabhan* S, Jost* M, Drennan* CL, Elias-Arnanz* M. (2017) A new facet of vitamin B12: Gene regulation by cobalamin-based photoreceptors. Ann. Rev. Biochem. 86:485-514. Full text at Ann. Rev. Biochem.
Bridwell-Rabb* J, Drennan* CL. (2017) Vitamin B12 in the spotlight again. Curr. Op. Chem. Biol. 37:63-70. Full text at ScienceDirect
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Mattaini KR, Brignole EJ, Kini M, Davidson SM, Fiske BP, Drennan CL, Vander Heiden* MG. (2015) An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation. Cancer & Metabolism 3:5. Full text at Cancer & Metabolism
Funk MA, Marsh ENG, Drennan* CL. (2015) Substrate-bound structures of benzylsuccinate synthase reveal how toluene is activated in anaerobic hydrocarbon degradation. J. Biol. Chem. 290:22398-22408. Full text at JBC
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Gagnon DM, Brophy MB, Bowman SE, Stich TA, Drennan CL, Britt* RD, Nolan* EM. (2015). Manganese binding properties of human calprotectin under conditions of high and low calcium: X-ray crystallographic and advanced EPR spectroscopic analysis. Journal of the American Chemical Society 137(8):3004-3016. Full text at ACS Publications
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Funk MA, Judd ET, Marsh ENG, Elliott SJ, Drennan* CL. (2014) Structures of benzylsuccinate synthase elucidate roles of accessory subunits in glycyl radical enzyme activation and activity. Proc. Natl. Acad. Sci. U.S.A. 111(28):10161-10166. Full text at PNAS
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Goldman PJ, Grove TL, Sites LA, McLaughlin MI, Booker SJ, Drennan* CL. (2013). X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification. Proc. Natl. Acad. Sci. U.S.A. 110(21):8519-8524. Full text at PNAS
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Chang WC, Dey M, Liu P, Mansoorabadi SO, Moon S, Zhao ZK, Drennan CL, Liu* HW. (2013) Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction. Nature 496:114-118. Full text at Nature
Brignole‡ EJ, Ando‡ N, Zimanyi CM, Drennan* CL. (2012) The prototypic class Ia ribonucleotide reductase from Escherichia coli: Still surprising after all these years. Biochemical Society Transactions 40:523-530. Full text online
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Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan* CL. (2012) An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes. Chemistry & Biology 19:855-865. Full text at Cell Press
Aye Y, Brignole EJ, Long MJC, Chittuluru J, Drennan CL, Asturias FJ, Stubbe* J. (2012) Clofarabine targets the large subunit (alpha) of human ribonucleotide reductase in live cells by assembly into persistent hexamers. Chemistry & Biology 19:799-805. Full text at Cell Press
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Yun D, Dey M, Higgins LJ, Yan F, Liu H-W, Drennan* CL. (2011) Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis. J. Am. Chem. Soc. 131:11262-11269. Full text at ACS Publications
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Vey JL, Drennan* CL. (2011) Structural insights into radical generation by the radical SAM superfamily. Chem. Rev. 111:2487-2506. Full text at ACS Publications
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