Research in the Drennan Lab

Metalloproteins and Metalloenzymes

The primary targets of research in the Drennan lab are enzymes that contain metals or metallocofactors. These metalloenzymes use the enhanced reactivity of transition metals to catalyze challenging chemical reactions including radical-based chemistry and manipulation of organometallic bonds. The lab is also interested in metalloproteins that sense changes in the cellular environment or act as redox mediators.

 

A structural approach to studying enzymes

The Drennan lab combines X-ray crystallography with techniques from biochemistry and biophysics to understand enzyme mechanisms. We call like to call this approach “structural enzymology”.

Crystallography

Crystallography

Electron Microscopy

Electron Microscopy

Small-angle X-ray Scattering

Small-angle X-ray Scattering

Analytical Ultracentrifugation

Analytical Ultracentrifugation

 

 

 

At the atomic scale, we are interested in providing detailed three-dimensional information about the nature of complex metallocofactors to help understand how protein environment modulates reactivity. At the protein scale, we are interested in seeing how enzymes are constructed to control substrate access and specificity, and how they prevent loss of reactive intermediates or damage to expensive cofactors. At the largest scale, that of protein complexes, we want to know how proteins interact and how those interactions explain the observed behavior. Protein complexes are often large, have multiple distinct states, and can have large inter- and intrasubunit motions; therefore a single “snapshot” usually does not tell the entire story.

Current Projects

Metalloenzymes, Enzymes, & Medicine

Environmental Bioinorganic Chemistry

Metal Uptake & Metallochaperones